AHCY Protein (AA 1-432) (His tag)
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- Target See all AHCY Proteins
- AHCY (Adenosylhomocysteinase (AHCY))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-432
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This AHCY protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
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MSDKLPY KVADIGLAAW GRKALDIAEN EMPGLMRMRE RYSASKPLKG ARIAGCLHMT VETAVLIETL VTLGAEVQWS SCNIFSTQDH AAAAIAKAGI PVYAWKGETD EEYLWCIEQT LYFKDGPLNM ILDDGGDLTN LIHTKYPQLL PGIRGISEET TTGVHNLYKM MANGILKVPA INVNDSVTKS KFDNLYGCRE SLIDGIKRAT DVMIAGKVAV VAGYGDVGKG CAQALRGFGA RVIITEIDPI NALQAAMEGY EVTTMDEACQ EGNIFVTTTG CIDIILGRHF EQMKDDAIVC NIGHFDVEID VKWLNENAVE KVNIKPQVDR YRLKNGRRII LLAEGRLVNL GCAMGHPSFV MSNSFTNQVM AQIELWTHPD KYPVGVHFLP KKLDEAVAEA HLGKLNVKLT KLTEKQAQYL GMSCDGPFKP DHYRY - Purity
- > 90% by SDS-PAGE
- Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
- Top Product
- Discover our top product AHCY Protein
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Adenosylhomocysteinase (AHCY) (AA 1-432) protein (GST tag)
AHCY Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Adenosylhomocysteinase (AHCY) (AA 1-432) protein (His tag)AHCY Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Adenosylhomocysteinase (AHCY) (AA 1-432) protein (His tag)AHCY Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Adenosylhomocysteinase (AHCY) (AA 2-432) protein (His-SUMO Tag)AHCY Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Adenosylhomocysteinase (AHCY) (AA 1-448), (full length) protein (His tag)AHCY Origin: Saccharomyces cerevisiae Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Adenosylhomocysteinase (AHCY) protein (Myc-DYKDDDDK Tag)AHCY Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- AHCY (Adenosylhomocysteinase (AHCY))
- Alternative Name
- Adenosylhomocysteinease/AHCY (AHCY Products)
- Background
- AHCY, also known as adenosylhomocysteinase isoform 1, is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCYs activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease. It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S-adenosyl-L-methionine methyltransferases. Recombinant human AHCY protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 48.8 kDa (441aa)
- NCBI Accession
- NP_000678
- UniProt
- P23526
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