VAMP2 Protein (AA 1-94) (His tag)

Catalog No. ABIN7281268

This Recombinant VAMP2 protein is produced in Escherichia coli (E. coli).

Quick Overview for VAMP2 Protein (AA 1-94) (His tag) (ABIN7281268)

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Protein Characteristics: AA 1-94

Purification tag / Conjugate: This VAMP2 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSATAA TAPPAAPAGE GGPPAPPPNL TSNRRLQQTQ AQVDEVVDIM RVNVDKVLER DQKLSELDDR ADALQAGASQ FETSAAKLKR KYWWKNLK

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid In Phosphate buffered saline (  pH 7.4) containing 10 % Glycerol, 0.1  mM PMSF

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Alternative Name: VAMP2

Background: VAMP2, also known as vehicle-associated membrane protein 2 isoform 1, which is an integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. VAMP2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25. Recombinant human VAMP2 protein, fused to His-tag at N-terminus, was expressed in E,coli and using conventional chromatography.

Molecular Weight: 12.8 kDa (118aa), confirmed by MALDI-TOF

NCBI Accession: NP_055047

UniProt: P63027

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport