GDA Protein (AA 1-454) (His tag)
Quick Overview for GDA Protein (AA 1-454) (His tag) (ABIN5854994)
Target
See all GDA ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-454
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Purification tag / Conjugate
- This GDA protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMCAARTP PLALVFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE SSQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHAPQ YAFAGSNVDL PLLEWLNKYT FPTEQRFRST DVAEEVYTRV VRRTLKNGTT TACYFGTIHT DSSLILAEIT DKFGQRAFVG KVCMDLNDTV PEYKETTEES VKETERFVSE MLQKNYPRVK PIVTPRFTLS CTETLMSELG NIAKTHDLYI QSHISENREE IEAVKSLYPS YKNYTDVYDK NNLLTNKTVM AHGCYLSEEE LNIFSERGAS IAHCPNSNLS LSSGLLNVLE VLKHKVKIGL GTDVAGGYSY SMLDAIRRAV MVSNVLLINK VNEKNLTLKE VFRLATLGGS QALGLDSEIG NFEVGKEFDA LLINPRASDS PIDLFYGDFV GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV
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Biological Activity Comment
- Specific activity is > 4,000pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
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Guanine Deaminase (GDA) (AA 1-454) protein (His tag)
custom-made GDA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Guanine Deaminase (GDA) (AA 1-454) protein (His tag)custom-made GDA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Guanine Deaminase (GDA) (AA 1-454) protein (GST tag)GDA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Guanine Deaminase (GDA) (AA 1-454) (Active) proteinGDA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS, EAA Active
Guanine Deaminase (GDA) (AA 1-454) protein (His tag)GDA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Guanine Deaminase (GDA) proteinGDA Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl( pH 8.0) containing 0.15M NaCl, 1 mM DTT, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- GDA (Guanine Deaminase (GDA))
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Alternative Name
- Guanine deaminase/GDA
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Background
- Gda, also known as guanine deaminase, catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant mouse Gda, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.
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Molecular Weight
- 53.4 kDa (477aa) Confirmed by MALDI-TOF
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NCBI Accession
- NP_034396
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UniProt
- Q9R111
Target
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