MYDGF Protein (AA 25-166) (His tag)
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- Target See all MYDGF (D17Wsu104e) Proteins
- MYDGF (D17Wsu104e) (DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-166
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MYDGF protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
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VSEPTTV PFDVRPGGVV HSFSQDVGPG NKFTCTFTYA SQGGTNEQWQ MSLGTSEDSQ HFTCTIWRPQ GKSYLYFTQF KAELRGAEIE YAMAYSKAAF ERESDVPLKS EEFEVTKTAV SHRPGAFKAE LSKLVIVAKA ARSEL - Purity
- > 95% by SDS-PAGE
- Top Product
- Discover our top product D17Wsu104e Protein
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DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e) protein (His tag)
D17Wsu104e Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e) (AA 32-173) protein (His tag)D17Wsu104e Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl( pH 8.0) containing 0.1M NaCl, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- MYDGF (D17Wsu104e) (DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e))
- Alternative Name
- SF20/MYDGF (D17Wsu104e Products)
- Background
- Mydgf, also known as Myeloid-derived growth factor, is a bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and repair after myocardial infarction. It stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Mydgf-deficient mice develop larger infarct scars and more severe contractile dysfunction compared to wild-type mice. Recombinant mouse Mydgf, fused to His-tag at N-termineus, was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 18.1 kDa (165aa) Confirmed by MALDI-TOF
- NCBI Accession
- NP_543027
- UniProt
- Q9CPT4
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