MDH2 Protein (AA 25-338) (His tag)
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- Target See all MDH2 Proteins
- MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 25-338
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MDH2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVQSKET ECTYFSTPLL LGKKGLEKNL GIGKITPFEE KMIAEAIPEL KASIKKGEDF VKNMK
- Purity
- > 95% by SDS-PAGE
- Biological Activity Comment
- Specific activity is > 800unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C
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Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 25-338) protein (His tag)
MDH2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 25-338) protein (His-IF2DI Tag)MDH2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 1-338) protein (GST tag)MDH2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 25-338) protein (His-SUMO Tag)MDH2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) protein (Myc-DYKDDDDK Tag)MDH2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In Phosphate buffer saline ( pH 7.4) containing 20 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))
- Alternative Name
- Malate dehydrogenase 2/MDH2 (MDH2 Products)
- Background
- Mdh2, also known as Malate dehydrogenase 2. Mdh2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, Mdh2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant mouse Mdh2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 35.4 kDa (335aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_032643
- UniProt
- P08249
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