SERPINC1 Protein (AA 1-546) (His tag)

Catalog No. ABIN5855151

This Recombinant SERPINC1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for SERPINC1 Protein (AA 1-546) (His tag) (ABIN5855151)

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 90% by SDS-PAGE

Product Details

Protein Characteristics: AA 1-546

Purification tag / Conjugate: This SERPINC1 protein is labelled with His tag.

Sequence: ADPHGSPVDI CTAKPRDIPM NPMCIYRSPE KKATEDEGSE QKIPEATNRR VWELSKANSR FATTFYQHLA DSKNDNDNIF LSPLSISTAF AMTKLGACND TLQQLMEVFK FDTISEKTSD QIHFFFAKLN CRLYRKANKS SKLVSANRLF GDKSLTFNET YQDISELVYG AKLQPLDFKE NAEQSRAAIN KWVSNKTEGR ITDVIPSEAI NELTVLVLVN TIYFKGLWKS KFSPENTRKE LFYKADGESC SASMMYQEGK FRYRRVAEGT QVLELPFKGD DITMVLILPK PEKSLAKVEK ELTPEVLQEW LDELEEMMLV VHMPRFRIED GFSLKEQLQD MGLVDLFSPE KSKLPGIVAE GRDDLYVSDA FHKAFLEVNE EGSEAAASTA VVIAGRSLNP NRVTFKANRP FLVFIREVPL NTIIFMGRVA NPCVKHHHHH H

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 1nM.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Alternative Name: Serpin C1/Antithrombin-III

Background: SERPINC1, also known as antithrombin-III, is a member of the Serpin superfamily of the serine protease inhibitors. It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa. It is one of the most important coagulation inhibitors and is the basic enzyme for the therapeutic action of heparin. In common with SerpinA5 and D1, the inhibitory activity of SerpinC1 dramatically increases the presence of heparin and other glycosaminoglycans. It mediates the promotion of prostaglandin release, inhibitors of leukocyte activation and down-regulation of many inflammatory cytokines. This protein has anti-inflammatory properties besides anticoagulant. Recombinant human SERPINC1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 50.1 kDa (441aa)

NCBI Accession: NP_000479

UniProt: P01008