GSTM1 Protein (AA 1-218)

Catalog No. ABIN666743

This Recombinant GSTM1 protein is produced in Escherichia coli (E. coli).

Quick Overview for GSTM1 Protein (AA 1-218) (ABIN666743)

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-218

Sequence: MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK

Characteristics: GSTM1, 1-218 aa, Mouse, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 40,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) containing 5 mM glutathione

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Alternative Name: Glutathione S-transferase mu 1/GSTM1

Background: GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTM1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 25.9 kDa (218aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NCBI Accession: NP_034488

Pathways: Negative Regulation of Transporter Activity