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GSTA1 Protein (AA 1-222)

Recombinant GSTA1 protein expressed in Escherichia coli (E. coli).
Catalog No. ABIN666922

Quick Overview for GSTA1 Protein (AA 1-222) (ABIN666922)

Target

See all GSTA1 Proteins
GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 9
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Human

Source

  • 14
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  • 1
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity

> 90 %
  • Protein Characteristics

    AA 1-222

    Sequence

    MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF

    Characteristics

    GSTA1, 1-222aa, Human, E.coli

    Purification

    > 90% by SDS-PAGE

    Endotoxin Level

    < 1 EU per 1ug of protein (determined by LAL method)

    Biological Activity Comment

    Specific activity is > 35,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
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  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT 10 % glycerol

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
  • Target

    GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

    Alternative Name

    Glutathione S-transferase alpha 1/GSTA1

    Background

    GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

    Molecular Weight

    25.6 kDa (222aa) confirmed by MALDI-TOF

    NCBI Accession

    NP_665683
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