DLD Protein (AA 36-509) (His tag)
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- Target See all DLD Proteins
- DLD (Dihydrolipoamide Dehydrogenase (DLD))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 36-509
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DLD protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- DLD, 36-509aa, Human, His tag, E.coli
- Purity
- > 95 % by SDS - PAGE
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Dihydrolipoamide Dehydrogenase (DLD) (AA 1-509) protein (GST tag)
DLD Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Dihydrolipoamide Dehydrogenase (DLD) proteinDLD Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Dihydrolipoamide Dehydrogenase (DLD) protein (Myc-DYKDDDDK Tag)DLD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol
- Storage
- 4 °C
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- Target
- DLD (Dihydrolipoamide Dehydrogenase (DLD))
- Alternative Name
- DLD (DLD Products)
- Background
- DLD (Dihydrolipoamide dehydrogenase), also known as GCSL (glycine cleavage system L protein), is a component of the glycine cleavage system as well as of the alpha ketoacid dehydrogenase complexes. DLD is a flavin-dependent oxidoreductase and functions as a component of the alpha-keto acid dehydrogenase, the pyruvate dehydrogenase, the alpha-ketoglutarate dehydrogenase, the branched-chain alpha-keto acid dehydrogenase and as the L protein in the mitochondrial glycine cleavage system. Mutations in DLD protein can result in MSUD (maple syrup urine disease) and congenital infantile lactic acidosis. Recombinant human DLD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: DLDH, E3, GCSL, LAD, PHE3, Dihydrolipoyl dehydrogenase, mitochondrial Diaphorase, Dihydrolipoamide dehydrogenase, Dihydrolipoyl dehydrogenase, Dihydrolipoyl dehydrogenase mitochondrial, DLD, E3 component of pyruvate dehydrogenase, E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex, Glycine cleavage system L protein, Glycine cleavage system protein L, Lipoamide reductase, Lipoyl dehydrogenase, PHE 3,. NCBI no.: NP_000099
- Molecular Weight
- 54.4 kDa (511aa)
- Pathways
- Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis
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