Sonic Hedgehog Protein (SHH) (AA 25-198) (His tag)

Catalog No. ABIN666981

Product Details

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Protein Characteristics: AA 25-198

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Sonic Hedgehog protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: SHH, 25-198 aa, Mouse, His-tagged, Recombinant, E.coli

Purity: > 95 % by SDS-PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT.

Storage: 4 °C

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: SHH (SHH Products)

Background: SHH is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and Indian hedgehog (IHH). This protien is the best studied ligand of the hedgehog signaling pathway. It plays a key role in regulating vertebrate organogenesis. SHH contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Recombinant mouse SHH protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Dsh, Hhg1, Hx, Hx13, Sonic hedgehog protein HHG 1, HHG1, HLP 3, HLP3, Holoprosencephaly 3, HPE 3, HPE3, MCOPCB5, SHH, SMMC I, SMMCI, Sonic Hedgehog (Drosophila) homolog, sonic hedgehog homolog (Drosophila), Sonic hedgehog homolog, TPT, TPTPS. NCBI no.: NP_033196

Molecular Weight: 20.8 kDa (183aa), confirmed by MALDI-TOF

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development