Sonic Hedgehog Protein (SHH) (AA 181-280) (GST tag)

Catalog No. ABIN1319947

This Recombinant Sonic Hedgehog protein is produced in Wheat germ.

Quick Overview for Sonic Hedgehog Protein (SHH) (AA 181-280) (GST tag) (ABIN1319947)

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 181-280

Purification tag / Conjugate: This Sonic Hedgehog protein is labelled with GST tag.

Purpose: SHH (Human) Recombinant Protein (Q01)

Sequence: IHCSVKAENS VAAKSGGCFP GSATVHLEQG GTKLVKDLSP GDRVLAADDQ GRLLYSDFLT FLDRDDGAKK VFYVIETREP RERLLLTAAH LLFVAPHNDS

Characteristics: Human SHH partial ORF ( NP_000184, 181 a.a. - 280 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: SHH

Background: Full Gene Name: sonic hedgehog homolog (Drosophila)
Synonyms: HHG1,HLP3,HPE3,MCOPCB5,SMMCI,TPT,TPTPS

Gene ID: 6469

NCBI Accession: NM_000193

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development