Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

NFNB (AA 1-217) protein (His tag)

Origin: E. coli Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Catalog No. ABIN667164
  • Target
    NFNB
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-217
    Origin
    E. coli
    Source
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    His tag
    Application
    SDS-PAGE (SDS)
    Characteristics
    NFNB, 1-217aa, E.coli, His tag, E.coli
    Purity
    > 95 % by SDS - PAGE
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/ml (determined by Bradford assay)
    Buffer
    Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM Nacl, 1mM DTT, 10% glycerol
    Storage
    4 °C
  • Target
    NFNB
    Background
    NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: dprA, nfsB, nfsI, ntr, dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive. NCBI no.: NP_415110
    Molecular Weight
    26.0 kDa (237aa) confirmed by MALDI-TOF
You are here:
Support