MYL9 Protein (AA 1-172) (His tag)

Catalog No. ABIN667358

Product Details

Target: MYL9 (Myosin Regulatory Light Polypeptide 9 (MYL9))

Protein Type: Recombinant

Protein Characteristics: AA 1-172

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MYL9 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MSSKRAKAKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDEYL EGMMSEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEASGFIHE DHLRELLTTM GDRFTDEEVD EMYREAPIDK KGNFNYVEFT RILKHGAKDK DD

Characteristics: MYL9, 1-172aa, Human, His tag, E.coli

Purity: > 90% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: MYL9 (Myosin Regulatory Light Polypeptide 9 (MYL9))

Alternative Name: MYL9 (MYL9 Products)

Background: MYL9, also known as MLC2, is one of the numerous regulatory myosin light chains. Regulatory myosin light chains regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of regulatory myosin light chains is catalyzed by MLCK in the presence of calcium and calmodulin and it increases the actin-activated myosin ATPase activity, thereby regulates the contractile activity. Recombinant human MYL9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 21.9 kDa (192aa) confirmed by MALDI-TOF

NCBI Accession: NP_006088