AnsA (AA 1-338) protein (His tag)

Catalog No. ABIN667462

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for AnsA (AA 1-338) protein (His tag) (ABIN667462)

Target: AnsA

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Protein Characteristics: AA 1-338

Purification tag / Conjugate: His tag

Characteristics: ansA, 1-338aa, E.coli, His tag, E.coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT.

Storage: 4 °C

Target Details

Target: AnsA

Background: AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E.coli ansA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Cytoplasmic L-asparaginase I, ECK1765, JW1756. NCBI no.: NP_416281

Molecular Weight: 39.3 kDa (358aa), confirmed by MALDI-TOF