GPX1(U49C) (AA 1-203) protein (His tag)

Catalog No. ABIN667598

This Recombinant protein is produced in Escherichia coli (E. coli).

Quick Overview for GPX1(U49C) (AA 1-203) protein (His tag) (ABIN667598)

Target: GPX1(U49C)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 % by SDS - PAGE

Product Details

Protein Characteristics: AA 1-203

Purification tag / Conjugate: His tag

Characteristics: GPX1(U49C), 1-203aa, Human, His tag, E.coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 100mM NaCl

Storage: 4 °C

Target Details

Target: GPX1(U49C)

Alternative Name: GPX1 (U49C)

Background: GPX1 (Glutathione peroxidase 1) belongs to the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. The GPX1 is part of the enzymatic antioxidant defence, preventing oxidative damage to DNA, proteins and lipids by detoxifying hydrogen and lipid peroxides that may contribute to prostate cancer development. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX1(U49C) protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Glutathione peroxidase 1, GSHPX1. NCBI no.: NP_000572

Molecular Weight: 24.2kDa (223aa), confirmed by MALDI-TOF