Seryl-tRNA Synthetase (SARS) (AA 1-514) protein (His tag)
Quick Overview for Seryl-tRNA Synthetase (SARS) (AA 1-514) protein (His tag) (ABIN667644)
Target
See all Seryl-tRNA Synthetase (SARS) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-514
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Purification tag / Conjugate
- His tag
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Sequence
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MVLDLDL FRVDKGGDPA LIRETQEKRF KDPGLVDQLV KADSEWRRCR FRADNLNKLK NLCSKTIGEK MKKKEPVGDD ESVPENVLSF DDLTADALAN LKVSQIKKVR LLIDEAILKC DAERIKLEAE RFENLREIGN LLHPSVPISN DEDVDNKVER IWGDCTVRKK YSHVDLVVMV DGFEGEKGAV VAGSRGYFLK GVLVFLEQAL IQYALRTLGS RGYIPIYTPF FMRKEVMQEV AQLSQFDEEL YKVIGKGSEK SDDNSYDEKY LIATSEQPIA ALHRDEWLRP EDLPIKYAGL STCFRQEVGS HGRDTRGIFR VHQFEKIEQF VYSSPHDNKS WEMFEEMITT AEEFYQSLGI PYHIVNIVSG SLNHAASKKL DLEAWFPGSG AFRELVSCSN CTDYQARRLR IRYGQTKKMM DKVEFVHMLN ATMCATTRTI CAILENYQTE KGITVPEKLK EFMPPGLQEL IPFVKPAPIE QEPSKKQKKQ HEGSKKKAAA RDVTLENRLQ NMEVTDA -
Characteristics
- SARS, 1-514aa, Human, His tag, E.coli
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Seryl-tRNA Synthetase (SARS) (AA 1-514) protein (His tag)
custom-made SARS Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Seryl-tRNA Synthetase (SARS) (AA 1-512) protein (His tag)custom-made SARS Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Seryl-tRNA Synthetase (SARS) (AA 1-514) protein (GST tag)SARS Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Seryl-tRNA Synthetase (SARS) (AA 2-233), (partial) protein (GST tag)SARS Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Seryl-tRNA Synthetase (SARS) protein (Myc-DYKDDDDK Tag)SARS Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Seryl-tRNA Synthetase (SARS)
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Alternative Name
- SARS
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Background
- SARS (Seryl-tRNA synthetase, cytoplasmic) belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. This enzyme catalyzes the attachment of serine to tRNA (Ser). It is probably able to aminoacylate tRNA (Sec) with serine, to form the misacylated tRNA L-seryl-tRNA (Sec), which will be further converted into selenocysteinyl-tRNA (Sec). Recombinant human SARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 61.2 kDa (537aa)
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NCBI Accession
- NP_006504
Target
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