THEM2 Protein (AA 1-140) (His tag)

Catalog No. ABIN667825

Recombinant THEM2 protein expressed in Escherichia coli (E. coli).

Quick Overview for THEM2 Protein (AA 1-140) (His tag) (ABIN667825)

Target: THEM2 (ACOT13) (Acyl-CoA Thioesterase 13 (ACOT13))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95% by SDS-PAGE

Product Details

Protein Characteristics: AA 1-140

Purification tag / Conjugate: This THEM2 protein is labelled with His tag.

Sequence: MTSMTQSLRE VIKAMTKARN FERVLGKITL VSAAPGKVIC EMKVEEEHTN AIGTLHGGLT ATLVDNISTM ALLCTERGAP GVSVDMNITY MSPAKLGEDI VITAHVLKQG KTLAFTSVDL TNKATGKLIA QGRHTKHLGN

Characteristics: ACOT13, 1-140aa, Human, His tag, E.coli

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: THEM2 (ACOT13) (Acyl-CoA Thioesterase 13 (ACOT13))

Alternative Name: ACOT13

Background: Acyl-coenzyme A thioesterase 13, also known ACOT13, belongs to the thioesterase subfamily of esterase family. Highly expressed in kidney with moderate expression in brain, liver and intestines, ACOT13 contains a hotdog-fold and is thought to co-localize with microtubules, possibly playing a role in cellular proliferation events. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Recombinant human ACOT13, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 17.1 kDa (160aa) confirmed by MALDI-TOF

NCBI Accession: NP_060943