MGLL Protein (AA 1-313) (His tag)
Quick Overview for MGLL Protein (AA 1-313) (His tag) (ABIN7279205)
Target
See all MGLL ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-313
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Purification tag / Conjugate
- This MGLL protein is labelled with His tag.
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Sequence
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METGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDL LVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFA GMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFG IQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTN SVFHEINMWV SQRTATAGTA SPP -
Characteristics
- MGLL, 1-313aa, Human, His tag, E.coli
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Monoglyceride Lipase (MGLL) (AA 1-303) protein (His tag)
custom-made MGLL Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Monoglyceride Lipase (MGLL) (AA 1-303) protein (His tag)custom-made MGLL Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Monoglyceride Lipase (MGLL) (AA 1-313) protein (GST tag)MGLL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Monoglyceride Lipase (MGLL) (AA 1-333) protein (His tag)MGLL Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Monoglyceride Lipase (MGLL) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)MGLL Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- MGLL (Monoglyceride Lipase (MGLL))
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Alternative Name
- Monoglyceride Lipase/MGLL
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Background
- MGLL is membrane-associated member of the serine hydrolase superfamily. MGLL functions together with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides. It is most abundantly expressed in skeletal muscle and adipose tissue. Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molecular Weight
- 36.4 kDa (333aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_009214
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Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling, Lipid Metabolism
Target
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