NXT1 Protein (AA 1-140) (His tag)

Catalog No. ABIN667905

This Recombinant NXT1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for NXT1 Protein (AA 1-140) (His tag) (ABIN667905)

Target: NXT1 (NTF2-Related Export Protein 1 (NXT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 85% by SDS-PAGE

Product Details

Protein Characteristics: AA 1-140

Purification tag / Conjugate: This NXT1 protein is labelled with His tag.

Sequence: MASVDFKTYV DQACRAAEEF VNVYYTTMDK RRRLLSRLYM GTATLVWNGN AVSGQESLSE FFEMLPSSEF QISVVDCQPV HDEATPSQTT VLVVICGSVK FEGNKQRDFN QNFILTAQAS PSNTVWKIAS DCFRFQDWAS

Characteristics: NXT1, 1-140aa, Human, His tag, E.coli

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: NXT1 (NTF2-Related Export Protein 1 (NXT1))

Alternative Name: NXT1

Background: NXT1 functions as a nuclear export factor in both RAN (Ras-related nuclear protein) and CRM1 (required for chromosome region maintenance) dependent pathways. It is found to stimulate the export of u1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. This protien heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. Recombinant human NXT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molecular Weight: 18 kDa (160aa) confirmed by MALDI-TOF

NCBI Accession: NP_037380