RNMT Protein (AA 1-476) (His tag)
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- Target See all RNMT Proteins
- RNMT (RNA Guanine-7 Methyltransferase (RNMT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-476
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RNMT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
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MANSAKAEEY EKMSLEQAKA SVNSETESSF NINENTTASG TGLSEKTSVC RQVDIARKRK EFEDDLVKES SSCGKDTPSK KRKLDPEIVP EEKDCGDAEG NSKKRKRETE DVPKDKSSTG DGTQNKRKIA LEDVPEKQKN LEEGHSSTVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSVKQCQQRY EDMKNRRDSE YIFSAEFITA DSSKELLIDK FRDPQMCFDI CSCQFVCHYS FESYEQADMM LRNACERLSP GGYFIGTTPN SFELIRRLEA SETESFGNEI YTVKFQKKGD YPLFGCKYDF NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYE EKIKNNENKM LLKRMQALEP YPANESSKLV SEKVDDYEHA AKYMKNSQVR LPLGTLSKSE WEATSIYLVF AFEKQQ - Characteristics
- RNMT, 1-476aa, Human, His tag, E.coli
- Purity
- > 90% by SDS-PAGE
- Top Product
- Discover our top product RNMT Protein
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RNA Guanine-7 Methyltransferase (RNMT) (AA 1-476) protein (His tag)
custom-made RNMT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
RNA Guanine-7 Methyltransferase (RNMT) (AA 1-465) protein (His tag)custom-made RNMT Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
RNA Guanine-7 Methyltransferase (RNMT) (AA 31-126) protein (His-IF2DI Tag)RNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
RNA Guanine-7 Methyltransferase (RNMT) (AA 1-476) protein (GST tag)RNMT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
RNA Guanine-7 Methyltransferase (RNMT) protein (Myc-DYKDDDDK Tag)RNMT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- RNMT (RNA Guanine-7 Methyltransferase (RNMT))
- Alternative Name
- RNMT (RNMT Products)
- Background
- RNMT (mRNA cap guanine-N7 methyltransferase), also known as MET, RG7MT1 or hCMT1c, is a widely expressed nuclear protein that belongs to the mRNA cap methyltransferase family. Cap-dependent mRNA translation requires the methylation of the mRNA guanosine cap by RNMT. RNMT catalyzes the transfer of a methyl group from AdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7 position, thereby producing AdoHyc (S-adenosylhomocysteine) and m7GpppN terminated RNA. Recombinant human RNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 57 kDa (496aa)
- NCBI Accession
- NP_003790
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