AP1S2 Protein (AA 1-157) (His tag)
-
- Target See all AP1S2 Proteins
- AP1S2 (Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-157
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This AP1S2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MQFMLLFSRQ GKLRLQKWYV PLSDKEKKKI TRELVQTVLA RKPKMCSFLE WRDLKIVYKR YASLYFCCAI EDQDNELITL EIIHRYVELL DKYFGSVCEL DIIFNFEKAY FILDEFLLGG EVQETSKKNV LKAIEQADLL QEEAETPRSV LEEIGLT
- Characteristics
- AP1S2, 1-157aa, Human, His tag, E.coli
- Purity
- > 85% by SDS-PAGE
- Top Product
- Discover our top product AP1S2 Protein
-
Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) (AA 1-160) protein (His tag)
custom-made AP1S2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) (AA 1-157) protein (His tag)custom-made AP1S2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) (AA 1-157) protein (His tag)AP1S2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) (AA 1-157) protein (GST tag)AP1S2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl,2mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
-
- Target
- AP1S2 (Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2))
- Alternative Name
- AP1S2 (AP1S2 Products)
- Background
- AP1S2, also known as AP-1 complex subunit sigma-2, serves as the small subunit of AP-1 complex 1 and is a member of the adaptin protein family. Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. Recombinant human AP1S2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
- Molecular Weight
- 20.7 kDa (177aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_003907
-
