POMC Protein (AA 27-267) (His tag)

Catalog No. ABIN667920

This Recombinant POMC protein is expressed in Escherichia coli (E. coli).

Quick Overview for POMC Protein (AA 27-267) (His tag) (ABIN667920)

Target: POMC (Proopiomelanocortin (POMC))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 85% by SDS-PAGE

Product Details

Protein Characteristics: AA 27-267

Purification tag / Conjugate: This POMC protein is labelled with His tag.

Sequence: WCLESSQCQ DLTTESNLLE CIRACKPDLS AETPMFPGNG DEQPLTENPR KYVMGHFRWD RFGRRNSSSS GSSGAGQKRE DVSAGEDCGP LPEGGPEPRS DGAKPGPREG KRSYSMEHFR WGKPVGKKRR PVKVYPNGAE DESAEAFPLE FKRELTGQRL REGDGPDGPA DDGAGAQADL EHSLLVAAEK KDEGPYRMEH FRWGSPPKDK RYGGFMTSEK SQTPLVTLFK NAIIKNAYKK GE

Characteristics: POMC, 27-267aa, Human, His tag, E.coli

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: POMC (Proopiomelanocortin (POMC))

Alternative Name: POMC

Background: Pro-opiomelanocortin preproprotein, also known as POMC, a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. It regulates the corticosteroid production in the adrenal cortex. Also, POMC is cleaved into ten hormone chains named NPP, gamma-MSH, ACTH, alpha-MSH, CLIP, Lipotropin beta, Lipotropin gamma, beta-MSH, beta endorphin and Met-enkephalin. Defects in the gene that encodes POMC are the cause of POMC deficiency, which is characterized by red hair and adrenal insufficiency. Recombinant human POMC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 28.9 kDa (262aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NCBI Accession: NP_001030333

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour