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CBP Protein (GST tag)

CREBBP Origin: Human Host: Escherichia coli (E. coli) Recombinant >90% WB
Rockland
Catalog No. ABIN6699729
Supplier Product No.: 009-001-r59s
  • Target See all CBP (CREBBP) Proteins
    CBP (CREBBP) (CREB Binding Protein (CREBBP))
    Protein Type
    Recombinant
    Origin
    • 5
    • 3
    • 2
    Human
    Source
    • 7
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This CBP protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-r59s
    Supplier
    Rockland
    Purpose
    CBP recombinant protein-GST fusion protein
    Purification
    Recombinant human CBP (1319-1710) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
    Purity
    >90%
    Top Product
    Discover our top product CREBBP Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: CBP Protein is suitable for use in Western Blot. Expect a band approximately ~ 71 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 μg/μL
    Buffer
    CBP Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    CBP (CREBBP) (CREB Binding Protein (CREBBP))
    Alternative Name
    CREBBP (CREBBP Products)
    Background

    Synonyms: CREB Binding Protein, CREBBP, RTS, RSTS, CBP

    Background: CBP or CREB-binding protein is a nuclear transcriptional coactivator protein that binds specifically to the PKA-phosphorylated form of the CREB protein. Microinjection of an anti-CBP antiserum into fibroblasts leads to inhibition of transcription from a cAMP promoter (1). CBP can also cooperates with upstream activators, such as JUN. When JUN is phosphorylated at the transcriptionally stimulatory sites ser73 and ser63, it binds CBP with comparable affinity to CREB. Insulin signaling may directly regulate many cAMP signaling pathways at the transcriptional level by controlling CBP recruitment (2). Mutant CBP can be aberrantly recruited to CREB protein, resulting in inappropriate activation of gluconeogenesis and glucose intolerance. CBP Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway,and PKA/PKC Pathway research.

    NCBI Accession
    NM_004380
    Pathways
    TCR Signaling, Interferon-gamma Pathway, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
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