CBP Protein (GST tag)

Catalog No. ABIN6699731

Supplier Product No.: 009-001-s26s

Product Details

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This CBP protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-s26s

Supplier: Rockland

Purpose: KAT3A (CREBBP) recombinant protein-GST fusion protein

Purification: Recombinant human KAT3A (518-1207) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Purity: >90%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: KAT3A Protein is suitable for use in Western Blot. Expect a band approximately ~65 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: KAT3A Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Alternative Name: CREBBP (CREBBP Products)

Background:

Synonyms: CREBBP, CBP, KAT3A, RSTS, CREB-binding protein

Background: KAT3A has intrinsic histone acetyltransferase activity that acts as a scaffold to stabilize additional protein interactions with the transcription complex and acetylates both histone and non-histone proteins. KAT3A (CREBBP) expressed as a nuclear protein that binds to cAMP-response element binding protein (CREB) and involved in the transcriptional coactivation of many different transcription factors. KAT3A plays a main role in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition (1). KAT3A also plays a critical role in the transmission of inductive signals from cell surface receptors to the transcriptional apparatus (2). KAT3A Protein is ideal for investigators involved in Signaling Proteins, Acetyl/Methyltransferase Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, and PKA/PKC Pathway research.

NCBI Accession: NM_004380

Pathways: TCR Signaling, Interferon-gamma Pathway, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha