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FADD Protein (His tag)

FADD Origin: Human Host: Escherichia coli (E. coli) Recombinant >95% SDS, WB
Rockland
Catalog No. ABIN6699826
Supplier Product No.: 009-001-r92s
  • Target See all FADD Proteins
    FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))
    Protein Type
    Recombinant
    Origin
    • 9
    • 4
    Human
    Source
    • 7
    • 3
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This FADD protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB)
    Supplier Product No.
    009-001-r92s
    Supplier
    Rockland
    Purpose
    FADD recombinant protein-HIS Epitope
    Purification
    Recombinant full-length human FADD was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
    Purity
    >95%
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    Discover our top product FADD Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: FADD Protein is suitable for use in Western Blot. Expect a band approximately ~ 27 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 μg/μL
    Buffer
    FADD Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))
    Alternative Name
    FADD (FADD Products)
    Background

    Synonyms: GIG3, MORT1, MGC8528, FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD

    Background: FADD or Fas-Associated protein with Death Domain is an adaptor molecule that mediates death signaling by the Fas-receptor, tumor necrosis factor receptor and TRAIL-receptor. FADD binds to these receptors via the C-terminus Death Domain which then unmasks the N-terminal effector domain of FADD thereby allowing it to recruit caspase-8 and activate the cysteine protease cascade leading to apoptosis (1). Cells lacking FADD are defective in intracellular double-stranded RNA (dsRNA)-activated gene expression, including production of type I (alpha/beta) interferons and are thus very susceptible to viral infection (2). FADD Protein is ideal for investigators involved in Signaling Proteins, Adaptor Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, and NfkB Pathway research.

    NCBI Accession
    NM_003824
    Pathways
    Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
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