FADD Protein (His tag)
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- Target See all FADD Proteins
- FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This FADD protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB)
- Supplier Product No.
- 009-001-r92s
- Supplier
- Rockland
- Purpose
- FADD recombinant protein-HIS Epitope
- Purification
- Recombinant full-length human FADD was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
- Purity
- >95%
- Top Product
- Discover our top product FADD Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: FADD Protein is suitable for use in Western Blot. Expect a band approximately ~ 27 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- FADD Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))
- Alternative Name
- FADD (FADD Products)
- Background
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Synonyms: GIG3, MORT1, MGC8528, FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD
Background: FADD or Fas-Associated protein with Death Domain is an adaptor molecule that mediates death signaling by the Fas-receptor, tumor necrosis factor receptor and TRAIL-receptor. FADD binds to these receptors via the C-terminus Death Domain which then unmasks the N-terminal effector domain of FADD thereby allowing it to recruit caspase-8 and activate the cysteine protease cascade leading to apoptosis (1). Cells lacking FADD are defective in intracellular double-stranded RNA (dsRNA)-activated gene expression, including production of type I (alpha/beta) interferons and are thus very susceptible to viral infection (2). FADD Protein is ideal for investigators involved in Signaling Proteins, Adaptor Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, and NfkB Pathway research.
- NCBI Accession
- NM_003824
- Pathways
- Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
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