Follistatin Protein (FST)

Catalog No. ABIN6699879

Supplier Product No.: 009-001-u88-0020

Recombinant Follistatin protein expressed in Escherichia coli (E. coli).

Quick Overview for Follistatin Protein (FST) (ABIN6699879)

Target: Follistatin (FST)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 009-001-u88-0020

Supplier: Rockland

Purpose: Human Follistatin Recombinant Protein

Purification: Follistatin purity was determined to be greater than 95% as determined by analysis by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent neutralization of 7.5 ng/mL human Activin A. Complete neutralization is typically reached at less than 0.3 ug/mL.

Application Details

Application Notes:

Application Note: Follistatin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Follistatin in immunological assays. This product is provided as a lyophilized powder, in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer: This product is provided as a lyophilized powder, in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: Follistatin (FST)

Alternative Name: FST

Background:

Synonyms: FS, activin-binding protein, FSH-suppressing protein (FSP)

Background: Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-beta family members (activin, BMPs, myostatin, GDF-11 and TGF-beta 1). Mechanistically, follistatin works as an antagonist by complexing with TGF-beta family members to prevent them from interacting with their signaling receptors. Recombinant human Follistatin is a non-glycosylated protein, containing 289 amino acids, with a molecular weight of 31.7 kDa.

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion