Quick Overview for IL-33 Protein (ABIN6700128)
Target
See all IL-33 (IL33) ProteinsProtein Type
Origin
Source
Application
Purity
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Supplier Product No.
- 010-001-b50-0010
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Supplier
- Rockland
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Purpose
- Mouse Interleukin-33 Recombinant Protein
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Purification
- Interleukin-33 purity was determined to be greater than 90% as determined by reducing and non-reducing SDS-pAGE.
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Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the ability to induce the proliferation of D10S cells and is typically less than 1 ng/mL.
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Interleukin 33 (IL33) (AA 112-270) protein
IL33 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 33 (IL33) (AA 1-266) protein (His tag)custom-made IL33 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Interleukin 33 (IL33) (AA 1-270) protein (His tag)custom-made IL33 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Interleukin 33 (IL33) (AA 109-270) (Active) protein (His tag)IL33 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 33 (IL33) (AA 109-264) protein (His tag)IL33 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 33 (IL33) (AA 109-270) protein (His tag)IL33 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 33 (IL33) protein (His tag)IL33 Origin: Rhesus Monkey Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 33 (IL33) (AA 171-269) protein (GST tag)IL33 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Application Notes
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Application Note: Interleukin-33 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-33 in immunological assays.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution_Buffer: 0.02M HCl
Reconstitution_Volume: 10 μL (10-100 μL)
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Buffer
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Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
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Preservative
- Without preservative
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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Expiry Date
- 6 months
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- IL-33 (IL33) (Interleukin 33 (IL33))
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Alternative Name
- Il33
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Background
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Synonyms: NP-HEV
Background: Interleukin 33 (IL-33) is a member of the IL-1 cytokine family and is constitutively expressed in smooth muscle cells and the airway epithelium. IL-33 signals through receptors, IL-1RL1 and IL-1RAP, to induce type 2 cytokine production in polarized Th2 cells. Recombinant mouse IL-33 is a non-glycosylated protein, containing 159 amino acids, with a molecular weight of 17.7 kDa.
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UniProt
- Q8BVZ5
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Pathways
- Production of Molecular Mediator of Immune Response
Target
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