KDM2A Protein (GST tag)

Catalog No. ABIN6700235

Supplier Product No.: 009-001-s32s

Product Details

Target: KDM2A (Lysine (K)-Specific Demethylase 2A (KDM2A))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This KDM2A protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-s32s

Supplier: Rockland

Purpose: KDM2A recombinant protein-GST fusion protein

Purification: Recombinant human KDM2A (1-748) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Purity: >90%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: KDM2A Protein is suitable for use in Western Blot. Expect a band approximately ~115 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: KDM2A is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: KDM2A (Lysine (K)-Specific Demethylase 2A (KDM2A))

Alternative Name: KDM2A (KDM2A Products)

Background:

Synonyms: CXXC8, FBL11, FBL7, FBXL11, JHDM1A, LILINA, Lysine-specific demethylase 2A, EC 1.14.11.27, CXXC-type zinc finger protein 8, F-box and leucine-rich repeat protein 11, F-box protein FBL7, F-box protein Lilina, F-box/LRR-repeat protein 11, JmjC domain-containing histone demethylation protein 1A, [Histone-H3]-lysine-36 demethylase 1A

Background: KDM2A or lysine (K)-specific demethylase 2A is a member of the F-box protein family that are components of the modular E3 ubiquitin protein ligases called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination (1). KDM2A belongs to the Fbls class and, in addition to the presence of an F-box, contains at least six highly degenerated leucine-rich repeats which play a role in epigenetic silencing. The demethylase activity of the JmjC domain-containing proteins is conserved from yeast to human (2). KDM2A Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.

NCBI Accession: NM_012308

Pathways: Warburg Effect