KDM4A Protein (GST tag)

Catalog No. ABIN6700237

Supplier Product No.: 009-001-s33s

This Recombinant KDM4A protein is produced in Insect cells (Sf9).

Quick Overview for KDM4A Protein (GST tag) (ABIN6700237)

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Purification tag / Conjugate: This KDM4A protein is labelled with GST tag.

Supplier Product No.: 009-001-s33s

Supplier: Rockland

Purpose: KDM4A recombinant protein-GST fusion protein

Purification: Recombinant human KDM4A (1-886) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: KDM4A Protein is suitable for use in Western Blot. Expect a band approximately ~150 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: KDM4A is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Alternative Name: KDM4A

Background:

Synonyms: JMJD2A, JHDM3A, KIAA0677, Lysine-specific demethylase 4A, EC 1.14.11.-, JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A

Background: KDM4A or lysine (K)-specific demethylase 4A is a member of the Jumonji domain 2 (JMJD2) family that encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. KDM4A functions in euchromatin to remove histone methylation marks that are associated with active transcription (1). KDM4A also act as a trimethylation-specific demethylase those converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor (2). KDM4A Protein is ideal for investigators involved in Cancer, Cell Cycle, and Inflammation research.

Pathways: Warburg Effect