LOXL2 Protein (GST tag)

Catalog No. ABIN6700249

Supplier Product No.: 009-001-s41s

Product Details

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This LOXL2 protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-s41s

Supplier: Rockland

Purpose: LOXL2 recombinant protein-GST fusion protein

Purification: Recombinant human LOXL2 (519-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.

Purity: >75%

Application Details

Application Notes:

Application Note: LOXL2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.LOXL2 Protein is suitable for use in Western Blot. Expect a band approximately ~65 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: LOXL2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Alternative Name: LOXL2 (LOXL2 Products)

Background:

Synonyms: LOR2, WS9-14, Lysyl oxidase homolog 2, EC 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14

Background: LOXL2 is a member of the lysyl oxidase gene family which is essential for the biogenesis of connective tissue. LOXL2 encodes an extracellular copper-dependent amine oxidase that catalyzes the first step in the formation of crosslinks in collagens and elastin. LOXL2 may play an important role in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family (1). LOXL2 may mediate fibroblast activation through enzymatic crosslinking of fibrillar collagen and local matrix tension, resulting in activation of TGFb1 signaling (2). LOXL2 Protein is ideal for investigators involved in Signaling Proteins, Lysyl Oxidase Proteins, Cancer, and Metabolic Disorder research.

Pathways: Chromatin Binding