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SARS-CoV-2 Spike S1 protein (His tag) Protein

Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Func, SDS
Pubmed (9)
Catalog No. ABIN6952427
$418.00
Plus shipping costs $45.00
100 μg ABIN6952427
100 μg ABIN6952427
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  • Target
    SARS-CoV-2 Spike S1
    Protein Type
    Recombinant
    Origin
    • 70
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 61
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike S1 protein is labelled with His tag.
    Application
    Functional Studies (Func), SDS-PAGE (SDS)
    Sequence
    AA 16-685
    Characteristics
    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76.9 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    AA 16-685
    Purity
    >90 % as determined by SDS-PAGE.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized 2019-nCoV S1 protein, His Tag at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 2-20 ng/mL (QC tested).
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Lucas, Klein, Sundaram, Liu, Wong, Silva, Mao, Oh, Mohanty, Huang, Tokuyama, Lu, Venkataraman, Park, Israelow, Vogels, Muenker, Chang, Casanovas-Massana, Moore, Zell, Fournier, Wyllie, Campbell, Lee et al.: "Delayed production of neutralizing antibodies correlates with fatal COVID-19. ..." in: Nature medicine, 2021 (PubMed).

    Song, Bartley, Chow, Ngo, Jiang, Zamecnik, Dandekar, Loudermilk, Dai, Liu, Sunshine, Liu, Wu, Hawes, Alvarenga, Huynh, McAlpine, Rahman, Geng, Chiarella, Goldman-Israelow, Vogels, Grubaugh et al.: "Divergent and self-reactive immune responses in the CNS of COVID-19 patients with neurological symptoms. ..." in: Cell reports. Medicine, pp. 100288, 2021 (PubMed).

    Maritz, Woudberg, Bennett, Soares, Lapierre, Devine, Kimberg, Bouic: "Validation of high-throughput, semiquantitative solid phase SARS coronavirus-2 serology assays in serum and dried blood spot matrices." in: Bioanalysis, 2021 (PubMed).

    Mazzini, Martinuzzi, Hyseni, Benincasa, Molesti, Casa, Lapini, Piu, Trombetta, Marchi, Razzano, Manenti, Montomoli: "Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples." in: Journal of immunological methods, Vol. 489, pp. 112937, 2021 (PubMed).

    Haslwanter, Dieterle, Wec, OBrien, Sakharkar, Florez, Tong, Rappazzo, Lasso, Vergnolle, Wirchnianski, Bortz, Laudermilch, Fels, Mengotto, Malonis, Georgiev, Quiroz, Wrapp, Wang, Dye, Barnhill, Dye et al.: "A Combination of Receptor-Binding Domain and N-Terminal Domain Neutralizing Antibodies Limits the Generation of SARS-CoV-2 Spike Neutralization-Escape Mutants. ..." in: mBio, Vol. 12, Issue 5, pp. e0247321, 2021 (PubMed).

    Christopher Minteer, Arnau Casanovas-Massana, Tao Li, David McDonald, Linda Wang, Si Hui Pan, David Caianiello, Jesse Collinski, Edward deRamon, Robert Hale, Rebecca Howell, Jason Ray, Joseph Vinetz, Morgan Levine, Albert Ko, David Spiegel: "Multi-site Validation of a SARS-CoV-2 IgG/IgM Rapid Antibody Detection Kit" in: bioRxiv, 2020

    Pierce, Preston-Hurlburt, Dai, Aschner, Cheshenko, Galen, Garforth, Herrera, Jangra, Morano, Orner, Sy, Chandran, Dziura, Almo, Ring, Keller, Herold, Herold: "Immune responses to SARS-CoV-2 infection in hospitalized pediatric and adult patients." in: Science translational medicine, Vol. 12, Issue 564, 2020 (PubMed).

    Luetkens, Metcalf, Planelles, Zheng, Larragoite, Spivak, Spivak, Steinbach, Blaylock, Avila, Hankey, Martins, Slev, Mannuel, Sajadi, Rapoport, Atanackovic: "Successful transfer of anti-SARS-CoV-2 immunity using convalescent plasma in an MM patient with hypogammaglobulinemia and COVID-19." in: Blood advances, Vol. 4, Issue 19, pp. 4864-4868, 2020 (PubMed).

    Wei, Wan, Yan, Wang, Zhang, Yang, Zhang, Fan, Li, Deng, Sun, Gong, Yang, Wang, Wang, Li, Yang, Li, Zhang, Wang, Du, Zong, Yin, Zhang, Wang, Peng, Lin, Feng, Qin, Chen, Gao, Zhang, Cao, Zhong: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." in: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, 2020 (PubMed).

  • Target
    SARS-CoV-2 Spike S1
    Synonyms
    E2, Surface Glycoprotein, S
    Target Type
    Viral Protein
    Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
    Molecular Weight
    76.9 kDa
    Gene ID
    43740568
    UniProt
    P0DTC2
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