SARS-CoV-2 Spike S1 Protein (AA 14-683, P.1 - gamma, P.1.1 - Gamma, P.1.2 - Gamma) (His tag)

Catalog No. ABIN7274778

This Recombinant SARS-CoV-2 Spike S1 protein is produced in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike S1 Protein (AA 14-683, P.1 - gamma, P.1.1 - Gamma, P.1.2 - Gamma) (His tag) (ABIN7274778)

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 14-683, P.1 - gamma, P.1.1 - Gamma, P.1.2 - Gamma

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with His tag.

Purpose: SARS-CoV-2 Spike S1 (Gamma P.1/P.1.1/P.1.2) Protein

Sequence: Gln14-Arg683(L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y)

Characteristics: Recombinant SARS-CoV-2 Spike S1 (Gamma P.1/P.1.1/P.1.2) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln14-Arg683(L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-CoV-2 Spike S1 (Gamma P.1/P.1.1/P.1.2) , His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA.,SARS-CoV-2 Spike S1 (Gamma P.1/P.1.1/P.1.2) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SARS-CoV-2 Spike S1 (Gamma P.1/P.1.1/P.1.2) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike S1

Target Type: Viral Protein

Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 76.11 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.