SARS-CoV-2 Spike S1 Protein (AA 14-683, D614G) (His-Avi Tag)

Catalog No. ABIN7274404

This Recombinant SARS-CoV-2 Spike S1 protein is expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike S1 Protein (AA 14-683, D614G) (His-Avi Tag) (ABIN7274404)

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 14-683, D614G

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated SARS-COV-2 Spike S1 (D614G) Protein

Sequence: Gln14-Arg683(D614G)

Characteristics: Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683(D614G).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2ug/ml (100ul/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.,SARS-COV-2 Spike S1 (D614G) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SARS-COV-2 Spike S1 (D614G) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike S1

Target Type: Viral Protein

Background: The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.

Molecular Weight: 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.