SARS-CoV-2 NSP1 Protein (His tag)

Catalog No. ABIN6952638

This Recombinant SARS-CoV-2 NSP1 protein is produced in Escherichia coli (E. coli).

Quick Overview for SARS-CoV-2 NSP1 Protein (His tag) (ABIN6952638)

Target: SARS-CoV-2 NSP1 (NSP1) (SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1))

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This SARS-CoV-2 NSP1 protein is labelled with His tag.

Purpose: SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag

Sequence: AA 1-180

Characteristics: SARS-CoV-2 NSP1, His Tag is expressed from E.coli cells. It contains AA Met 1 - Gly 180 (Accession # YP_009725297.1). Predicted N-terminus: Met
This protein carries a polyhistidine tag at the C-terminus.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, Arginine, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

Target Details

Target: SARS-CoV-2 NSP1 (NSP1) (SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1))

Alternative Name: SARS-CoV-2 Host Translation Inhibitor Nsp1

Target Type: Viral Protein

Background: The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.

Molecular Weight: 21.7 kDa

NCBI Accession: YP_009725297