SARS-CoV-2 Envelope Protein (SARS-CoV-2 E) (His tag,GST tag)
SARS-CoV-2 E
Origin: SARS Coronavirus-2 (SARS-CoV-2)
Host: Escherichia coli (E. coli)
Recombinant
>90 % as determined by SDS-PAGE.
SDS
1 image
(2 references)-
- Target See all SARS-CoV-2 Envelope (SARS-CoV-2 E) products
- SARS-CoV-2 Envelope (SARS-CoV-2 E)
- Protein Type
- Recombinant
- Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SARS-CoV-2 Envelope protein is labelled with His tag,GST tag.
- Application
- SDS-PAGE (SDS)
- Purpose
- SARS-CoV-2 (COVID-19) Envelope protein, His Tag
- Sequence
- AA 1-75
- Characteristics
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SARS-CoV-2 Envelope protein, GST,His Tag (ENN-C5128) is expressed from E.coli cells. It contains AA Met 1 - Val 75 (Accession # QHU79206.1). Predicted N-terminus: Met
This protein carries a GST tag at the N-terminus and a polyhistidine tag at the C-terminus. - Purity
- >90 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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SARS-CoV-2 Envelope (SARS-CoV-2 E) (AA 1-75) (Active) protein (His-Avi Tag)
SARS-CoV-2 E Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE. SDS Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 5 mM EDTA, Arginine, pH 8.0
- Handling Advice
- Please avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For long term storage, the product should be stored at lyophilized state at -20°C or lower.
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: "Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin." in: bioRxiv : the preprint server for biology, (2021) (PubMed).
: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).
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: "Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin." in: bioRxiv : the preprint server for biology, (2021) (PubMed).
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- Target
- SARS-CoV-2 Envelope (SARS-CoV-2 E)
- Alternative Name
- SARS-CoV-2 Envelope Protein (SARS-CoV-2 E Products)
- Target Type
- Viral Protein
- Background
- The SARS-CoV-2 is composed of a double-layered lipid envelope, including Spike glycoprotein (S), Envelope protein (E), Membrane glycoprotein (M), and Nucleocapsid protein (Nucleocapsid protein, N). Among them, The amino acid sequence of the SARS-CoV-2 envelope protein is 95 % identical of the SARS envelope protein.
- Molecular Weight
- 36.8 kDa
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