SARS-CoV-2 Envelope Protein (SARS-CoV-2 E) (AA 1-75) (His-Avi Tag)
This Recombinant SARS-CoV-2 Envelope protein is produced in Escherichia coli (E. coli).
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Catalog No. ABIN6952737
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Quick Overview for SARS-CoV-2 Envelope Protein (SARS-CoV-2 E) (AA 1-75) (His-Avi Tag) (ABIN6952737)
Target
SARS-CoV-2 Envelope (SARS-CoV-2 E)
Protein Type
Recombinant
Biological Activity
Active
Origin
SARS Coronavirus-2 (SARS-CoV-2)
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
Purity
> 95 % by SDS-PAGE.
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Protein Characteristics
- AA 1-75
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Purification tag / Conjugate
- This SARS-CoV-2 Envelope protein is labelled with His-Avi Tag.
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Sequence
- MYSFVSEETG TLIVNSVLLF LAFVVFLLVT LAILTALRLC AYCCNIVNVS LVKPSFYVYS RVKNLNSSRV PDLLV
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Specificity
- Met1-Val75
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Characteristics
- Recombinant 2019-nCoV envelope Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Val75) of 2019-ncov envelope (Accession #QHD43418.1) fused with a 6xHis,Avi tag at the N-terminus.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Envelope at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid with a linear range of 1.2-41.1 ng/mL.
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SARS-CoV-2 Envelope (SARS-CoV-2 E) protein (His tag,GST tag)
SARS-CoV-2 E Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: Escherichia coli (E. coli) Recombinant >90 % as determined by SDS-PAGE. SDS
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied as a 0.22 μm filtered solution in 20 mM Tris,250 mM NaCl,0.5 % TritonX-100, pH 8.0.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -80 °C
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Storage Comment
- This product is stable at ≤ -70°C for up to 6 months from the date of receipt.|For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
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- SARS-CoV-2 Envelope (SARS-CoV-2 E)
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Alternative Name
- SARS-CoV-2 Envelope Protein
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Target Type
- Viral Protein
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Background
- 2019-nCoV E protein,2019-nCoV sM protein,Envelope protein,Env polyprotein,Envelope glycoprotein,env,COVID-19,E
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Gene ID
- 43740570
Target
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