SIRPA Protein (AA 31-373) (Fc-His Tag)

Catalog No. ABIN6961082

Product Details

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Protein Type: Recombinant

Protein Characteristics: AA 31-373

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SIRPA protein is labelled with Fc-His Tag.

Application: ELISA

Purpose: Recombinant Human SIRPa with C-terminal human Fc and 6xHis tag

Specificity: SIRPa (Glu31-Tyr373) hFc (Glu99-Ala330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: SIRPalpha (SIRPA Products)

Background: Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Molecular Weight: predicted molecular mass of 70-98 kDa after removal of the signal peptide.

Gene ID: 140885

UniProt: P78324