Protein L Protein (AA 106-470) (His tag,AVI tag,Biotin)

Catalog No. ABIN7013589

This Recombinant Protein L protein is expressed in Escherichia coli (E. coli).

Quick Overview for Protein L Protein (AA 106-470) (His tag,AVI tag,Biotin) (ABIN7013589)

Target: Protein L

Protein Type: Recombinant

Biological Activity: Active

Origin: Peptostreptococcus magnus

Source: Escherichia coli (E. coli)

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 106-470

Purification tag / Conjugate: This Protein L protein is labelled with His tag,AVI tag,Biotin.

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: Biotinylated Recombinant Protein L Protein, His,Avitag™ (MALS & FACS verified)

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: MALS & FACS verified

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Target Details

Target: Protein L

Background: Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Molecular Weight: 43.7 kDa