LRRC15 Protein (His-Avi Tag,Biotin)

Catalog No. ABIN7199082

Product Details

Target: LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))

Protein Type: Recombinant

Origin: Cynomolgus, Rhesus Monkey

Source: HEK-293 Cells

Purification tag / Conjugate: This LRRC15 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Cynomolgus / Rhesus macaque LRRC15 / LIB Protein, His,Avitag™

Sequence: Tyr 22 - Gly 538

Characteristics: Biotinylated Cynomolgus / Rhesus macaque LRRC15, His,Avitag (LR5-C82E7) is expressed from human 293 cells (HEK293). It contains AA Tyr 22 - Gly 538 (Accession # A0A2K5UKB6-1).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 61.6 kDa. The protein migrates as 70-90 kDa under reducing (R) condition due to glycosylation.

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))

Alternative Name: LRRC15 / LIB (LRRC15 Products)

Background: Synonyms: Leucine-rich repeat-containing protein 15,LRRC15,LIB,hLib,
Description: LRRC15 (Leucine-rich repeat-containing protein 15) is also known as LIB and hLib. LRRC15 is highly expressed in a variety of solid tumors. LRRC15 was expressed on stromal fibroblasts in many solid tumors (e.g., breast, head and neck, lung, pancreatic) as well as directly on a subset of cancer cells of mesenchymal origin (e.g., sarcoma, melanoma, glioblastoma). LRRC15 expression was induced by TGFβ on activated fibroblasts (αSMA+) and on mesenchymal stem cells. These collective findings suggested LRRC15 as a novel CAF and mesenchymal marker with utility as a therapeutic target for the treatment of cancers with LRRC15-positive stromal desmoplasia or cancers of mesenchymal origin. ABBV-085 is a monomethyl auristatin E (MMAE)-containing antibody-drug conjugate (ADC) directed against LRRC15, and it demonstrated robust preclinical efficacy against LRRC15 stromal-positive/cancer-negative, and LRRC15 cancer-positive models as a monotherapy, or in combination with standard-of-care therapies.

Molecular Weight: 61.6 kDa