SPAM1 Protein

Catalog No. ABIN7199566

Recombinant SPAM1 protein expressed in HEK-293 Cells.

Quick Overview for SPAM1 Protein (ABIN7199566)

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Grade: MALS verified

Product Details

Purpose: Human PH20 / SPAM1 Protein, Tag Free (MALS verified)

Sequence: Leu 36 - Ser 490

Characteristics: Human PH20, Tag Free (PH0-H5219) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Ser 490 (Accession # P38567-1).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries no "tag". The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-70 kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM NaCl, pH 7.5

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Alternative Name: / SPAM1

Background: Synonyms: SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15,
Description: Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N - acetyl - beta - D - glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Molecular Weight: 52.6 kDa

NCBI Accession: NP_694859