CD109 Protein (CD109) (His-Avi Tag,Biotin)
Quick Overview for CD109 Protein (CD109) (His-Avi Tag,Biotin) (ABIN7199130)
Target
See all CD109 ProteinsProtein Type
Origin
Source
Purity
Grade
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Purification tag / Conjugate
- This CD109 protein is labelled with His-Avi Tag,Biotin.
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Purpose
- Biotinylated Human CD109 / CPAMD7 Protein, His,Avitag™ (MALS & SPR verified)
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Sequence
- Val 22 - Ser 1269
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Characteristics
- Biotinylated Human CD109, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ser 1269 (Accession # Q6YHK3-1).
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Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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CD109 (CD109) protein
CD109 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
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Application Notes
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 143.5 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.
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Comment
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- PBS, pH 7.4
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Storage
- -20 °C
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Storage Comment
- -20°C
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- CD109
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Alternative Name
- CD109 / CPAMD7
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Background
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Synonyms: CD109,CPAMD7,r150,p180,CPAMD7,
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Molecular Weight
- 143.5 kDa
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NCBI Accession
- NP_598000
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Pathways
- Activated T Cell Proliferation
Target
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