FGFR2 beta protein (His tag)

Catalog No. ABIN7125938

Recombinant protein expressed in HEK-293 Cells.

Quick Overview for FGFR2 beta protein (His tag) (ABIN7125938)

Target: FGFR2 beta

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: His tag

Purpose: Cynomolgus FGF R2 (IIIb) protein, His Tag

Sequence: Pro 154 - Lys 368

Characteristics: Cynomolgus FGFR2 (IIIb), His Tag is expressed from human 293 cells (HEK293). It contains AA Pro 154 - Lys 368 (Accession # A0A2K5TL84-1).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.9 kDa. The protein migrates as 43-55 kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: FGFR2 beta

Alternative Name: FGF R2 (IIIb) protein

Background: Synonyms: FGF R2 (IIIb),FGFR2B,FGFR2,
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.

Molecular Weight: 25.9 kDa