14-3-3 theta Protein (YWHAQ) (GST tag)

Catalog No. ABIN7317745

This Recombinant 14-3-3 theta protein is produced in Escherichia coli (E. coli).

Quick Overview for 14-3-3 theta Protein (YWHAQ) (GST tag) (ABIN7317745)

Target: 14-3-3 theta (YWHAQ)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 88 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This 14-3-3 theta protein is labelled with GST tag.

Purpose: Recombinant Human 14-3-3 tau/14-3-3 theta/YWHAQ Protein (GST Tag)

Sequence: Met 1-Asn 245

Characteristics: A DNA sequence encoding the human YWHAQ (P27348) (Met 1-Asn 245) was fused with the GST tag at the N-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 0.15m NaCl, 20 mM GSH, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3 tau/14-3-3 theta/YWHAQ

Background:

Background: G protein-coupled receptor kinase 5, also known as G protein-coupled receptor kinase GRK5 and GRK5, is a member of the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRKs specifically phosphorylate agonist-occupied G protein-coupled receptors at the inner surface of the plasma membrane (PM), leading to receptor desensitization. GRKs utilize a variety of mechanisms to bind tightly, and sometimes reversibly, to cellular membranes. GRKs play an important role in mediating agonist-specific desensitization of numerous G protein-coupled receptors. GRK5 contains one AGC-kinase C-terminal domain, one protein kinase domain and one RGS domain. GRK5 specifically phosphorylates the activated forms of G protein-coupled receptors. Phospholipid-stimulated autophosphorylation may represent a novel mechanism for membrane association and regulation of GRK5 activity. GRK5 deficiency significantly exaggerates microgliosis and astrogliosis in the presence of an inflammatory initiator, such as the excess fibrillar Abeta and the subsequent active inflammatory reactions. GRK5 deficiency has been linked to early Alzheimer's disease in humans and mouse models of the disease.

Synonym: 1C5;41701;HS1

Molecular Weight: 54.6 kDa

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction