ENPP7 Protein (His tag)

Catalog No. ABIN7194215

Recombinant ENPP7 protein expressed in HEK-293 Cells.

Quick Overview for ENPP7 Protein (His tag) (ABIN7194215)

Target: ENPP7 (Alkaline Sphingomyelinase (Alk-Smase))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This ENPP7 protein is labelled with His tag.

Purpose: Recombinant Mouse ENPP7/NPP-7 Protein (His Tag)

Sequence: Met 1-Gln 421

Characteristics: A DNA sequence encoding the mouse ENPP7 (Q3TIW9) (Met 1-Gln 421) was expressed, with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 7.6

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ENPP7 (Alkaline Sphingomyelinase (Alk-Smase))

Alternative Name: ENPP7/NPP-7

Background:

Background: Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.

Synonym: Alk-SMase;Gm254

Molecular Weight: 47 kDa

UniProt: Q3TIW9