Cadherin 5 Protein (CDH5) (His tag,Fc Tag)

Catalog No. ABIN7317868

This Recombinant Cadherin 5 protein is produced in HEK-293 Cells.

Quick Overview for Cadherin 5 Protein (CDH5) (His tag,Fc Tag) (ABIN7317868)

Target: Cadherin 5 (CDH5)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Cadherin 5 protein is labelled with His tag,Fc Tag.

Purpose: Recombinant Human VE-Cadherin/CDH5 Protein (His & Fc Tag)(Active)

Sequence: Met 1-Gln 593

Characteristics: A DNA sequence encoding the extracellular domain of human CDH5 (NP_001786.2) (Met 1-Gln 593) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 10E4 cells/well are added to Recombinant Human Cadherin-5 coated plates (0.8 μg/mL with 100 μL/well), approximately >50% will adhere after 1 hour at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Cadherin 5 (CDH5)

Alternative Name: VE-Cadherin/CDH5

Background:

Background: Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, an endothelial specific cell-cell adhesion molecule, plays a pivotal role in the formation, maturation and remodeling of the vascular wall. VE-Cadherin is widely considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. This specificity of VE-cadherin for vascular endothelial cells is important not only in blood and lymph vessel biology and medicine, but also for cell-type-based diagnoses, notably those of metastatic tumors. As a classical cadherin, VE-Cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Mechanisms that regulate VE-cadherin-mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. In addition to its adhesive functions, VE-Cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Consequently, VE-cadherin is essential during embryonic angiogenesis.

Synonym: 7B4,CD144

Molecular Weight: 92 kDa

NCBI Accession: NP_001786

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2