Casein Kinase 1 gamma 2 Protein (His tag)

Catalog No. ABIN7318105

This Recombinant Casein Kinase 1 gamma 2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Casein Kinase 1 gamma 2 Protein (His tag) (ABIN7318105)

Target: Casein Kinase 1 gamma 2 (CSNK1G2) (Casein Kinase 1, gamma 2 (CSNK1G2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Casein Kinase 1 gamma 2 protein is labelled with His tag.

Purpose: Recombinant Human CSNK1G2 Protein (His Tag)

Sequence: Met 18-Lys415

Characteristics: Recombinant Human Casein kinase I isoform gamma-2 is produced by our E.coli expression system and the target gene encoding Met18-Lys415 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,500 mM NaCl,10 % Glycerol,1 mM DTT, pH 8.0.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: Casein Kinase 1 gamma 2 (CSNK1G2) (Casein Kinase 1, gamma 2 (CSNK1G2))

Alternative Name: CSNK1G2

Background:

Background: Casein kinase I gamma 2 isoform (CSNK1G2), a member of the large casein kinase I (CKI) subfamily, protein kinase superfamily. It may affect the development of brain, and associate with vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. The CKI family includes several other isoforms (alpha, beta, gamma, and delta). Dishevelled (Dsh), another positive component of the Wnt pathway, becomes phosphorylated in response to Wnt signals. All the CKI isoforms, with the exception of gamma, increase the phosphorylation of Dsh in vivo. Casein kinase 1 gamma (CK1gamma, or CSNK1G) is associated with the cell membrane and binds to LRP. CK1gamma was found to be needed for Wnt signaling through Wnt receptor LRP. CSNK1G2 inhibits Smad3-mediated TGF-beta responses including induction of target genes and cell growth arrest, and this inhibition is dependent on CSNK1G2 kinase activity. The overexpression of CSNK1G2 in human cancers, may act as an oncoprotein during tumorigenesis. In addition, as an MTA1s-binding protein, CSNK1G2 could further potentiate the estrogen receptor (ER) corepressive function of MTA1s.

Synonym: Casein kinase I isoform gamma-2, CK1G2, CSNK1G2

Molecular Weight: 47.6 kDa

Pathways: Hedgehog Signaling