CD3 epsilon Protein (CD3E) (mFc Tag)

Catalog No. ABIN7319783

Product Details

Target: CD3 epsilon (CD3E)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This CD3 epsilon protein is labelled with mFc Tag.

Purpose: Recombinant Human CD3 epsilon/CD3E (C-mFC)

Sequence: Gln22-Thr48

Characteristics: Recombinant Human T-Cell Surface Glycoprotein CD3 Epsilon Chain is produced by our Mammalian expression system and the target gene encoding Gln22-Thr48 is expressed with a mFc tag at the C-terminus.

Purity: >95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized  Human  CD3E-mFc(Cat#C00E) at 10μg/ml (100 μl/well) can bind Human Anti-CD3(Cat#GMP-A018).  The ED50 of Human CD3E-mFc(Cat#C00E) is 9.4 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD3 epsilon (CD3E)

Alternative Name: CD3 epsilon/CD3E (CD3E Products)

Background:

Background: T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Synonym: T-Cell Surface Glycoprotein CD3 Epsilon Chain, T-Cell Surface Antigen T3/Leu-4 Epsilon Chain, CD3e, CD3E, T3E

Molecular Weight: 28.5 kDa

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway