CCL5 Protein (His tag)

Catalog No. ABIN7321250

This Recombinant CCL5 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CCL5 Protein (His tag) (ABIN7321250)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CCL5 protein is labelled with His tag.

Purpose: Recombinant Rat CCL5/RANTES Protein (His Tag)

Sequence: Ser25-Ser92

Characteristics: Recombinant Rat C-C motif chemokine 5 is produced by our E.coli expression system and the target gene encoding Ser25-Ser92 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: <1.0 EU per μg as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, 2 mM EDTA, pH 7.4 .

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: CCL5/RANTES

Background:

Background: C-C motif chemokine 5(CCL5) is a β-chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes. CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5. Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28. Oligomerization of CCL5 on glycosaminoglycans is required for CCR1mediated leukocyte adhesion and activation as well as CCL5's interaction with the chemokine CXCL4/PF4.The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes.CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature.

Synonym: C-C motif chemokine 5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, Ccl5, Scya5

Molecular Weight: 10 kDa

UniProt: P50231

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration