DFFA Protein (His tag)

Catalog No. ABIN7318410

This Recombinant DFFA protein is expressed in Escherichia coli (E. coli).

Quick Overview for DFFA Protein (His tag) (ABIN7318410)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This DFFA protein is labelled with His tag.

Purpose: Recombinant Human DFFA Protein (His Tag)

Sequence: Met 1-Thr331

Characteristics: Recombinant Human DNA Fragmentation Factor Subunit alpha is produced by our E.coli expression system and the target gene encoding Met1-Thr331 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Alternative Name: DFFA

Background:

Background: DNA Fragmentation Factor Subunit Alpha (DFFA). DFFA exists as a heterodimer (DFF) with DFFB. DFF is activated once DFFA is cleaved by Caspase-3. The cleaved fragments of DFFA detach from DFFB (the active component of DFF), which in turn triggers DNA fragmentation as well as chromatin condensation during apoptosis. A reduced level of DFFA detected in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression.

Synonym: DNA Fragmentation Factor Subunit Alpha, DNA Fragmentation Factor 45 kDa Subunit, DFF-45, Inhibitor of CAD, ICAD, DFFA, DFF1, DFF45, H13

Molecular Weight: 38.7 kDa

UniProt: O00273

Pathways: Apoptosis, Caspase Cascade in Apoptosis