DDC Protein (His tag)
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- Target See all DDC Proteins
- DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This DDC protein is labelled with His tag.
- Purpose
- Recombinant Human DOPA Decarboxylase/DDC Protein (His Tag)(Active)
- Sequence
- Met 1-Glu 480
- Characteristics
- A DNA sequence encoding the full length of human DDC (NP_000781.1) (Met 1-Glu 480) was expressed with a polyhistidine tag at the C-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to 3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1000 pmoles/min/μg.
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Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC) (AA 1-480) protein (His tag)
custom-made DDC Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC) (AA 1-480) protein (His tag)custom-made DDC Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC) (AA 1-480) protein (GST tag)DDC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC) (AA 1-480) protein (His tag)DDC Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8, 10 % glycerol
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))
- Alternative Name
- DOPA Decarboxylase/DDC (DDC Products)
- Background
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Background: Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.
Synonym: AADC
- Molecular Weight
- 55 kDa
- NCBI Accession
- NP_000781
- Pathways
- Dopaminergic Neurogenesis
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